ABSTRACT
@#ObjectiveTo study the relationship between the antitumor immunoreaction and the cell apoptosis in esophageal carcinoma.MethodsThe IL-1β converting enzyme, Fas and FasL protein were labeled by LSAB immunohistochemistric method in 46 cases of esophageal carcinoma, of which 13 cases were labeled by TdT mediated dUTP nick end labeling (TUNEL).ResultsThe positive rates of ICE, Fas and FasL proteins in the cancer nests were 78.26%, 60.87% and 47.83%. The expression of ICE protein was related to the histological grading of the cancer.Conclusions The expression of Fas, FasL protein may be related to the immunological escape of the cancer cell; the expression of ICE was related to the histological grading of the cancer.
ABSTRACT
Purpose:To study the role of DCs in tumor infiltrating T lymphocytes activation of esophageal carcinomas.Methods:46 specimens of patients with esophageal carcinoma were used to investigate the DCs (CD1a), the subsets of tumor infiltrating T lymphoctyes (CD3,CD4,CD8, CD25) and costimulatory B7 using monoclonal antibodies by immunohistochemistry staining LSAB method and reverse transcription polymerase chain reaction (RT PCR). Results:There was significant correlation between CD1a(+) DCs and CD3(+),CD4(+) T lymphocytes, but no marked correlation between CD1a(+) DCs and CD8(+), CD25(+) T lymphocytes. No specific B7 mRNA expression was found in all specimens. Conclusions:DCs may not activate T lymphocytes efficiently. B7 gene was expressed at low level or deficiency in esophageal carcinoma may be one of the important reasons of failure to activate lymphocytes and induce the immunosuppression.